In situ forming chitosan-based hydrogel as a lung sealant for biological lung volume reduction

作     者：Titima Songkroh 谢红国 于炜婷 吕国军 刘袖洞 王林 孙广炜 徐小溪 马小军 

作者机构：Dalian Institute of Chemical Physics Chinese Academy of Sciences University of Chinese Academy of Sciences College of Environment and Chemical EngineeringDalian University Affiliated Zhongshan Hospital of Dalian University 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2015年第60卷第2期

页      面：235-240,I0002页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 10[医学] 

基　　金：supported by the University of Chinese Academy of Sciences(UCAS)and Royal Thai Government(Office of The Civil Service Commission OCSC)Scholarship(27012552) 

主　　题：Chitosan based hydrogels Biological lung volume reduction Lung sealants Emphysema 

摘      要：Biological lung volume reduction （BLVR） using lung sealant has received more attention recently as a new non-surgical approach to emphysema treatment. Many tissue sealants have been studied but only a few have been proposed for BLVR. In this work, we prepared in situ forming chitosan-based hydrogels （CSG） using covalent cross-linking of chitosan and genipin in the cooperation of ionic interaction between chitosan and sodium orthophosphate hydrate （Na3PO4.12H20） and characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and rheological methods. CSG showed short gelation time （8 min）, high swelling ratio （〉100 %） and non-toxicity （3T3 mouse fibroblast cell viability 〉80 %） under physiological conditions. The application of lung sealant for BLVR was tested in a Chinese dog and evaluated by chest computed tomography. After 3 weeks of the installation of CSG in bronchopulmonary segment, the gel formation was detected at a localized region of bronchi and the local atelectasis occurred. Our findings indicate that this chitosan-based hydrogel is a promising new candidate for use as a lung sealant for BLVR.