Qualitative and Quantitative Analysis of Alkaloids in Cortex Phellodendri by HPLC-ESI-MS/MS and HPLC-DAD

作     者：ZHU Shuang-lai DOU Sheng-shan LIU Xin-ru LIU Run-hui ZHANG Wei-dong HUANG Hong-lin ZHANG Yi HU Yao-hua WANG Shu-ping 

作者机构：School of Pharmacy Second Military Medical University Shanghai 200433 P. R. China School of Pharmacy Jiangxi University of Traditional Chinese Medicine Nanchang 330004 P. R. China School of Pharmacy Shanghai Jiaotong University Shanghai 200240 P. R. China School of Pharmacy Fujian University of Traditional Chinese Medicine Fuzhou 350108 P. R. China School of Pharmacy Chengdu University of Traditional Chinese Medicine Chengdu 610075 P. R. China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2011年第27卷第1期

页      面：38-44页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：Supported by the National Natural Science Foundation of China(No30725045) the Foundation of Eleventh Five-Year-Plan of China(No2008ZX09202-002) the Shanghai Leading Academic Discipline Project, China(NoB906) the Scientific Foundation of Shanghai City, China(No07DZ19702) 

主　　题：Cortex Phellodendri Alkaloid HPLC-ESI-MS/MS Quantitative analysis Qualitative analysis Principal component analysis 

摘      要：A combined method of high performance liquid chromatograph-elecrtrospray-ionization mass spectrometer（HPLC-ESI-MS/MS） coupled with a photodiode array detector（HPLC-DAD） and principal component analysis（PCA） was applied to the qualitative and quantitative analyses of alkaloids in Cortex Phellodendri（CP） samples, and to the differentiation of two species of CP, Cortex Phellodendri Chinensis（CPC） and Cortex Phellodendri Amurensis（CPA）. Twenty-two peaks appeared in the HPLC-MS base peak chromatogram of CP detected by the HPLC-ESI-MS/MS analysis, and the alkaloids were identified according to the MSn data, the known MS fragmentation rules and the literature data. Five alkaloids including berberine, palmatine, jatrorrhizine, phellodendrine and magnoflorine were simultaneously determinated by the HPLC-DAD. Berberine was the primary component in all CP samples, and the contents of berberine and palmatine were exploited to be two critical parameters for effective discrimination between the two species of CP. The average content of berberine in CPC（58.75 mg/g） was higher than that in CPA（9.16 mg/g）, while the content of palmatine was less, only 0.25 mg/g in CPC and 4.19 mg/g in CPA. With the use of PCA, samples datasets were separated successfully into two different clusters corresponding to the two species, and berberine, pahnatine, phellodendrine and magnoflorine contribute most to the above mentioned calssifying . The proposed method oroved to be a useful tool in the aualitv control of Chinese herbal medicines.