Polymorphisms of Epstein-Barr virus BHRF1 gene, a homologue of bcl-2

作     者：Yong-Zheng Jing Yun Wang Yu-Ping Jia Bing Luo 

作者机构：Department of Microbiology Qingdao University Medical College Qingdao Shandong 266021 P. R. China Department of Clinical Laboratory Municipal Hospital of Penglai Penglai Shandong 265600 P. R. China 

出 版 物：《Chinese Journal of Cancer》 (CHINESE JOURNAL OF CANCER)

年 卷 期：2010年第29卷第12期

页      面：1000-1005页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 071005[理学-微生物学] 090102[农学-作物遗传育种] 10[医学] 

基　　金：National Natural Science Foundation of China (No30740068) Natural Science Foundation of Shandong Province, China(No Y2008C90) 

主　　题：EB病毒 早期基因 同源基因 多态性 全国人民代表大会 基因突变 开放阅读框架 聚合酶链反应 

摘      要：Background and Objective: EBV BamHI fragment H rightward open reading frame 1 (BHRF1), the Epstein-Barr virus (EBV) early gene, is structurally and functionally homologous to the oncogene bcl-2 and may play an important role in the development of EBV-associated tumors. To characterize the polymorphisms of BHRF1 in EBV-associated tumors, we analyzed the sequences of BHRF1 in isolates from nasopharyngeal carcinoma (NPC) and EBV-associated gastric carcinoma (EBVaGC) biopsies as well as throat washing (TW) samples from healthy donors. Methods: BHRF1 DNA sequences were analyzed by polymerase chain reaction (PCR) and sequencing for 39 NPC samples, 40 EBVaGC samples, and 53 EBV-positive TW samples from healthy donors. The variants of BHRF1 gene were classified according to the signature changes. The EBV types 1 and 2 at nuclear antigen (EBNA) 3C locus were determined by PCR. Results: Compared with EBV standard cell line B95-8, all isolates carried a silent mutation at amino acid (AA) 80 (nucleotide 54616 T→C), the AA88 L→V mutation was found in most isolates, and the AA79 V→L mutation in a few isolates. Other mutations were sporadically distributed. Based on the mutations at AA88 and AA79, 3 distinct variants of BHRF1 genes, designated as 79V88V, 79L88L, and 79V88L, were identified. The 79V88V was the most common variant. The distribution of the BHRF1 variants among the NPC, EBVaGC, and TW samples was not significant. The corresponding regions of bcl-2 homologues were conserved in all isolates except for 3 samples. The distribution of BHRF1 variants in type 1 and type 2 strains was significant different (P 0.001, contingency coefficient was 0.554). Conclusions: The 79V88V is the dominant variant in NPC, EBVaGC, and TW samples from healthy donors and preferential linkages between BHRF1 and EBNA3C variants exist. Conserved BHRF1 in Bcl-2 homologous domains is helpful to remain the important role of BHRF1.