The unmediated choline sensor based on layered double hydroxides in hydrogen peroxide detection mode

作     者：DING ShouNian SHAN Dan YAO WenJuan XUE HuaiGuo SUN YueMing COSNIER Serge 

作者机构：School of Chemistry&Chemical EngineeringSoutheast UniversityNanjing 211189China Key Laboratory of Environmental Materials&Environmental Engineering of Jiangsu ProvinceYangzhou 225002China School of Chemistry&Chemical EngineeringYangzhou UniversityYangzhou 225002China Départment de Chimie Moléculaire UMR-5250ICMG FR-2607CNRS UniversitéJoseph FouierBP-53Grenoble 38041France 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2009年第52卷第12期

页      面：2281-2286页

核心收录：

学科分类：080202[工学-机械电子工程] 08[工学] 0802[工学-机械工程] 

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 20505014 & 20773108) Jiangsu Science and Technology Development Foundation (Grant No. BE2007014) Jiangsu Natural Science Foundation (Grant No. BK2007563) 

主　　题：layered double hydroxides clay choline oxidase choline biosensor 

摘      要：In this work,we have developed a novel choline biosensor on the basis of immobilization of choline oxidase (ChOx) by the attractive materials layered double hydroxides (LDHs). Amperometric detection of choline was evaluated by holding the modified electrode at 0.5 V (vs. SCE). Due to the special properties of LDHs ([Zn3-Al-Cl]),such as chemical inertness,high porosity,and swelling property,the [Zn3-Al-Cl]/ChOx modified electrode exhibited an enhanced analytical performance. The biosensor provided a linear response to choline over a concentration range from 3.7 × 10-6 to 6.3 × 10-4 M with a low detection limit of 3 × 10-7 M based on S/N=3. The apparent Michaelis-Menten constant was calculated to be 1.38 mM. In addition,the interaction between ChOx and LDHs has also been investigated using FT-IR spectroscopy.