Distinct effect of potassium 2-(l-hydroxypentyl) - benzoate on hippocampal neurons, synapses and dystrophic axons in APP/PS1 mice

作     者：HUANG Long-jian ZHANG Yong LAN Jia-qi WANG Xiao-liang PENG Ying 

作者机构：Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China 

出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)

年 卷 期：2018年第32卷第9期

页      面：691-692页

核心收录：

学科分类：10[医学] 

基　　金：National Natural Sciences Foundation of China(81473200 81673420) CAMS InnovationFund for Medical Sciences (2017-I2M-2-004) 

主　　题：potassium 2-(l-hydroxypentyl)-benzoate APP/PS1 mice hippocampal synapse dysfunction dystrophic axons 

摘      要：OBJECTIVE To study the protective effect of potassium 2-(l-hydroxypentyl)-benzoate(PHPB) on hippocampal neurons,synapses and dystrophic axons in APP/PS1 *** Ten-month-old male APP/PS1 transgenic mice and age-matched wild-type mice were randomly divided into three groups:wild-type group(WT Con group,n=10),APP/PS1 group(Tg Con group,n=10) and PHPB treated APP/PS1 group(PHPB group,n=10).PHPB group received 30 mg · kg-1 PHPB by oral gavage once daily for 3 *** Con group and Tg Con group received the same volume of *** months later,mice were sacrificed for biochemical and pathological testing such as transmission electron microscopy,Golgi staining and Western boltting *** Under the transmission electron microscope,most hippocampal neurons and subcel ular organel es in WT Con group exhibited normal ***,the degenerative changes were observed in Tg Con group such as nuclear fragmentation,mitochondrial swelling,ribosomes detachment and autophagic vacuoles *** hippocampal synapses number and the thickness of postsynaptic density(PSD) were significantly decreased in Tg Con group compared with the WT Con group(P0.05).After PHPB treatment,the degenerative changes in APP/PS1 mice were alleviated to some *** synapse number has been elevated significantly(P0.05) and the PSD has been thickened as *** staining showed that the spine density of secondary and tertiary apical dendritic branches was significantly decreased in CA1 and DG areas of Tg Con group(P0.05).Sholl analysis revealed a decrease of dendritic complexity in Tg Con group compared with WT Con group(P0.05).These abnormalities were alleviated to some extent after PHPB *** blotting study showed that the protein levels of synaptic marker PSD-95 and synaptophysin were significantly decreased in the hippocampus of Tg Con group(P0.05).A significant increase of PSD-95(P0.05) and a slight increase of SYP were observed after the PHPB t