Icariin ameliorates learning and memory function via improving cerebral glucose metabolism disorder in APP/PS1/Tau triple transgenic Alzheimer disease mice

作     者：ZHANG Ying YAN Fei CHEN Mei-xiang JIN Hai NIE Jing SHI Jing-shan JIN Feng 

作者机构：Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education Institute of Digestive Diseases of Affiliated Hospital Zunyi Medical University Zunyi 563006 China 

出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)

年 卷 期：2018年第32卷第9期

页      面：704-705页

核心收录：

学科分类：10[医学] 

基　　金：National Natural Science Foundation of China(81660599) Foundation of Zunyi Medical University (2013F-686 2013F-738) Postgraduate Education Foundation of Guizhou Province(KYJJ2017008) 

主　　题：icariin Alzheimer disease glucosemetabolism glucose transporter 1 

摘      要：OBJECTIVE To investigate the protective effect of icariin(ICA) on learning and memory function in APP/PS1/Tau triple transgenic Alzheimer disease mice(3×Tg-AD mice),and then to explore whether its mechanism is related to the improvement of brain glucose metabolism *** Three-month-old male 3 ×Tg-AD mice were randomly divided into three groups(n=10):3×Tg group,3×Tg+ICA low-dose group(30 mg·kg-1) and 3×Tg + ICA high-dose group(60 mg·kg-1).Age-matched male wild type(WT) mice were randomly divided into two groups(n=10):WT control group and WT+ICA60 mg·kg-1 *** in vehicle(0.5% Tween-80 in distilled water) was given orally once a day for five months in the 3×Tg+ICA groups.3×Tg and WT control group were given an equal volume *** water maze was used to detect the learning and memory function of *** glucose metabolism in 3×Tg mice was observed by 18 F-FDG microPET imaging *** staining and HE staining were used to evaluate the survival neurons in hippocampus of *** oxidase assay was used to detect glucose contents in cortex of *** protein expression of APP,Aβ1-40,Aβ1-42 and glucose transporter 1(GLUT1),and the phosphorylation level of tau protein at multiple sites in hippocampus were detected by Western *** Behavioral examination revealed a profound decrease learning and memory function,accompanied by a decrease in number of neuronal cells in 3×Tg-AD ***,the cerebral18 F-FDG uptake rate per gram tissue was reduced and the glucose contents in the cortex were increased in 3×Tg-AD *** addition,Western blotting analysis showed that the expression of APP,Aβ1-40,Aβ1-42 proteins and the levels of tau protein phosphorylation at Ser199/202 and PHF-1(Ser396/404) sites were increased significantly,followed by a decrease of GLUT1 expression in hippocampus of 3×Tg-AD *** of these changes in behavioral functions,neuronal loss and related protein expression were reversed when mice were treated with