Role of mesocorticolimbic DA system in reinstatement induced by cue

作     者：JING Man-yi HAN Xiao ZHAO Tai-yun LIU Wen-feng SONG Rui LI Jin 

作者机构：Institute of Pharmacology and Toxicology Academy of Military Medical Sciences Academy of Mililtary Sciences Beijing 100850 China 

出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)

年 卷 期：2018年第32卷第9期

页      面：716-717页

核心收录：

学科分类：10[医学] 

基　　金：National Key Research and Development (2016YFC0800907) Project 973 (2015CB553504) National Natural Science Foundation of China(81573405) Beijing Nova Program(xx2014A014) 

主　　题：dopamine ventral tegmental area nucleus accumbens core and shell medial prefrontal cortex self-stimulation reinstatement 

摘      要：OBJECTIVE Dopamine(DA)plays important roles in Pavlovian conditioning by mediating reward,learning and *** the conditioning stimulation(CS) is the most important inducement for reinstatement in *** present study investigated the specific role of the DA projections to nucleus accumbens(NAc) and medial prefrontal cortices(mPFC) from ventral tegmental area(VTA) in reinstatement induced by ***(1)Optogenetic intracranial self-stimulation and ***-Cre transgenic mice received an injection of adeno-associated viral vectors encoding channelrhodopsin2(ChR2) or control vector into the VTA resulting in the selective expression of these opsins in DA ***,we stimulated the VTA,NAc(core and shel) or mP FC [prelimbic cortex(PL) and infralimbic cortex(IL)] via an optical *** the reinforcement test,the mice with ChR2 learned instrumental responses corresponding to the delivery of photostimulation into the VTA with multiple frequencies and during time;in the reinstatement phase,stimulation of the DA projections to NAc(core or shell) or mPFC(IL and PL) from VTA to induce reinstatement after 2 weeks of extinction of self-stimulation.(2)Reinstatement in cocaine *** encoding ChR2 or hM4 Di were injected into VTA of DAT-Cre transgenic *** mice with ChR2 and hM4 Di in DA neurons were trained to establish self-administration of *** 2 weeks of extinction,laser stimulation of the DA projections to NAc(core or shel) or mP FC(IL and PL) was conducted to induce *** that,Clozapine was injected in NAc core to test the impacts of VTA-NAc core depression on the reinstatement induced by cue.(3) Photometry of VTA DA neurons in ***-Cre transgenic mice were received an injection of AAV-DIO-Gcamp6 m into *** cocaine self-administration and extinction,mice with Gcamp6 m were challenged by cue(paired with cocaine previously) and the photometry of VTA DA neurons was conducted dur