SEQUENCE CONSERVATION OF HUMAN CYTOMEGALOVIRUS UL140 OPEN READING FRAME IN CLINICAL STRAINS

作     者：Ying Qi Yan-ping Ma Rong He Zheng-rong Sun Yao-hua Ji Yu-jing Huang Qiang Ruan 

作者机构：Virus Laboratory Shengjing Hospital China Medical University Shenyang 110004 

出 版 物：《Chinese Medical Sciences Journal》 (中国医学科学杂志（英文版）)

年 卷 期：2008年第23卷第1期

页      面：23-27页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China(30170986) 

主　　题：human cytomegalovirus UL140 conservation 

摘      要：Objective To investigate the variability of human cytomegalovirus （HCMV） UL140 open reading flame （ORF） in clinical strains, and to explore the relationship between the variability of UL140 ORF and different symptoms of HC-MV infection. Methods HCMV UL140 ORF was amplified by polymerase chain reaction and sequenced selectedly in 30 clinical strains. Results UL140 ORF of all clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identities among all strains were 96.5% -100.0% and 95.2% -100. 0%, respectively. All of the nucleotide changes were substitutions. The post-translational modification sites were conserved. The result of phylogenetic tree showed that the strains did not cluster according to different clinical symptoms. Conclusion HCMV UL140 ORF in clinical strains is highly conserved, which may play an important role in HC-MV infection.