Determination of lisinopril using anion exchange chromatography with integrated pulsed amperometric detection

作     者：Yu Xiu Liu Dan Shou Mei Lan Chen Zhi Dong Chen Pei Min Zhang Yan Zhu 

作者机构：School of Chemistry and Chemical EngineeringChangzhou UniversityChangzhou 213164China Zhe Jiang Academy of Traditional Chinese MedicineHangzhou 310007China College of Biology and Environment EngineeringZhejiang Shuren UniversityHangzhou 310015China Department of ChemistryXixi CampusZhejiang UniversityHangzhou 310028China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2012年第23卷第3期

页      面：335-338页

核心收录：

学科分类：080603[工学-有色金属冶金] 08[工学] 0806[工学-冶金工程] 080401[工学-精密仪器及机械] 0804[工学-仪器科学与技术] 081102[工学-检测技术与自动化装置] 0811[工学-控制科学与工程] 

基　　金：supported by the National Natural Science Foundation of China(Nos20775070,and 20911140271) the Natural Science Foundation of Zhejiang Province(NoR4080124) 

主　　题：Lisinopril Integrated pulsed amperometry Anion exchange chromatography Angiotensin converting enzyme inhibitor Plasma 

摘      要：A rapid and practical method for direct detection of lisinopril in anion exchange chromatography（AEC） has been developed with integrated pulsed amperometric detection（IPAD）.Dionex AS 18（250 mm×2 mm） and AG 18（50 mm×2 mm） columns and 40 mmol/L NaOH solution were used for ***-step potential waveform parameters were optimized to maximize the signal-to-noise ratio（S/N）.Utilizing the optimized waveform,the repeatability（intra-day） precision and intermediate（inter-day） precision were obtained with relative standard deviation（RSD） of 0.74,0.93,*** limit of quantification（LOQ） and limit of detection（LOD） were found to be 0.37,0.12ng/mL,respectively,with the correlation coefficient of 0.9998 over concentration range 0.01-1μg/*** present method was successfully applied to the determination of lisinopril in human *** recoveries of plasma sample spiked by 0.2μg/mL,0.8μg/mL lisinopril were 98.31-103.23%with RSD of 1.41%, 0.61%,respectively.