Identification of Rice Al-responsive Genes by Semi-quantitative Polymerase Chain Reaction using Sulfite Reductase as a Novel Endogenous Control

作     者：Jianjun Zhang Ying Yin Yuqi Wang Xinxiang Peng 

作者机构：Laboratory of Molecular Plant Physiology College of Life Sciences South China Agricultural University Guangzhou 510642 China Key Laboratory of Plant Functional Genomics and Biotechnology Education Department of Guangdong Province College of LifeSciences South China Agricultural University Guangzhou 510642 China Laboratory of Biochemistry and Molecular Biology College of Life Sciences South China Agricultural University Guangzhou 510642 China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2010年第52卷第5期

页      面：505-514页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Supported by the National Natural Science Foundation of China(30700052) the Natural Science Foundation of Guangdong Province (0700680) the Doctoral Program of Guangdong Natural Science Foundation (4600-E09022) 

主　　题：POLYMERASE chain reaction RICE PLANT genetics GENE expression GENETIC engineering 

摘      要：Based on the evidence that Al resistance is an inducible process and rice is an Al-resistant crop, identification of Al-responsive genes from rice may help to further clone *** genes in plants. Semi-quantitative and real-time polymerase chain reaction （PCR） is widely applied in gene transcrip- tional analyses, particularly for those genes with low transcript abundance. Normalization with proper endogenous control （EC） genes is critical for these two approaches in terms of reliability and precision. We first noticed that the expression of several commonly-used EC genes was depressed under AI stress, while sulfite reductase gene （SR） was stable throughout the AI treatment. The reliability of SR as an EC gene was further tested by analyzing the expression of a number of genes in response to Al challenge. Except for the consistent results obtained for the four previously-identified genes, nine additional genes were newly defined as Al-responsive in this study. Collectively, our results suggest that SR can be used as a novel EC gene for semi-quantitative and real-time PCR analysis of Al responsive genes, and that activated transport of silicon and stimulated metabolism of carotenoid and terpenoid could be involved in Al resistance in rice plants.