Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid

作     者：YUE Hao CHAN Wan YU KongJie GUO Lin LIU Ning LIU HuWei CAI ZongWei 

作者机构：Department of ChemistryHong Kong Baptist UniversityHong Kong SARChina Changchun University of Chinese MedicineChangchun 130117China Fujian Entry-Exit Inspection and Quarantine BureauFuzhou 350001China Institute of Analytical ChemistryCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2009年第52卷第10期

页      面：1576-1582页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Supported by the Research Grant Council,University Grants Committee of Hong Kong (Grant No. HKBU2459/06M) the Health and Health Services Research Fund of Hong Kong (Grant No. 05060141) 

主　　题：aristolochic acid DNA adducts quantitative analysis short review 

摘      要：Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized.