The Calcineurin B-Like Calcium Sensors CBL1 and CBL9 Together with Their Interacting Protein Kinase ClPK26 Regulate the Arabidopsis NADPH Oxidase RBOHF
The Calcineurin B-Like Calcium Sensors CBL1 and CBL9 Together with Their Interacting Protein Kinase ClPK26 Regulate the Arabidopsis NADPH Oxidase RBOHF作者机构：Institut for Biologie und Biotechnologie der Pflanzen Universitāt Münster Schlossplatz 4 48149 MOnster Germany Department of Applied Biological Science Tokyo University of Science Noda Chiba 278-8510 Japan
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2013年第6卷第2期
页 面：559-569页
核心收录：
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090401[农学-植物病理学] 0902[农学-园艺学]
基 金：supported by grants from the DFG to J.K supported by a Ph.D. fellowship from the IMPRS-CEDAD Graduate School to P.M supported by in part by the Grants-inAid from the Ministry of Education, Culture, Sports, Science and Technology of Japan for Scientific Research on Innovative Areas supported by for Scientific Research B. to K.K supported by a postdoctoral fellowship from the Alexander von Humbold Foundation
主 题：Calcium ROS CBL CIPK NADPH oxidase RBOH signal transduction.
摘 要：Stimulus-specific accumulation of second messengers like reactive oxygen species （ROS） and Ca^＋ are central to many signaling and regulation processes in plants. However, mechanisms that govern the reciprocal interrelation of Ca^＋ and ROS signaling are only beginning to emerge. NADPH oxidases of the respiratory burst oxidase homolog （RBOH） family are critical components contributing to the generation of ROS while Calcineurin B-like （CBL） Ca^＋ sensor proteins together with their interacting kinases （CIPKs） have been shown to function in many Ca^＋- signaling processes. In this study, we identify direct functional interactions between both signaling systems. We report that the CBL-interacting pro- tein kinase ClPK26 specifically interacts with the N-terminal domain of RBOHF in yeast two-hybrid analyses and with the full-length RBOHF protein in plant cells. In addition, CIPK26 phosphorylates RBOHF in vitro and co-expression of either CBL1 or CBL9 with CIPK26 strongly enhances ROS production by RBOHF in HEK293T cells. Together, these findings identify a direct interconnection between CBL-ClPK-mediated Ca^＋ signaling and ROS signaling in plants and provide evidence for a synergistic activation of the NADPH oxidase RBOHF by direct Ca^＋-binding to its EF-hands and Ca2＋-induced phospho-rylation by CBL1/9-ClPK26 complexes.
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