Electromagnetic Stimulation Combined with Aloe vera Increases Collagen Reorganization in Burn Repair

作     者：Sofia Poletti Leticia D. Lucke Fernanda O Bortolazzo Renata M. Acunha Marcos G. Mattos Femanda OG. Gaspi Thiago A. M. Andrade Maria Esmeria C. Amaral Andrea A .Aro Edson R Pimentel Marcelo AM. Esquisatto Fernanda A. S. Mendonga Glaucia M. T. Santos 

作者机构：Graduate Program in Biomedical Sciences of the Herminio Ometto University Center UIVIARARAS Araras Sao Paulo13607-339 Brazil Department of Structural and Functional Biology Institute of Biology University of Campinas-UNICAMP Campinas Sao Paulo13083-970 Brazil 

出 版 物：《Journal of Pharmacy and Pharmacology》 (药剂与药理学（英文版）)

年 卷 期：2018年第6卷第7期

页      面：633-646页

学科分类：0821[工学-纺织科学与工程] 0905[农学-畜牧学] 08[工学] 09[农学] 082102[工学-纺织材料与纺织品设计] 090501[农学-动物遗传育种与繁殖] 

主　　题：Burns repair herbal medicine electromagnetic stimulation rats 

摘      要：Objectives： Burns are shown as a clinical problem for their severity and multiple complications due to the time required to heal. Therapies that improve their healing are of great importance, especially for being minimally invasive, of low cost and best performance, all related to the speed and quality of healing. This study investigated the effects of the magnetic electro stimulator Haihuá CD9 isolated or in association with Aloe vera in rats skin burns. Methods： Experimental groups （n = 30/group） were： （C） Carbopol gel; （F） A. vera/Carbopol gel; （H） Haihuá＋Carbopol gel; （H＋F） Haihuá＋A. vera/Carbopol gel. Samples were collected on the 7th, 14th, and 21st experimental days for structural and morphometric analysis, hydroxyproline and glycosaminoglycans quantification, zymography for MMP-2 and MMP-9 and Western Blotting for TGF-β1, VEGF, Collagen I and III. Key findings： The expression of TGF-β1 in H＋F was increased on the 7th day and of MMP-9 on the 7th and 14th days. The expression of VEGF increased in the first experimental periods and decreased in the last for the treated groups. There was an increase in the fibroblasts and birefringent collagen fibers in groups treated with Haihuá isolated or in association with A. vera in all periods. The quantification of collagen I increased, while collagen III decreased in H＋F. The higher amount of GAGs and MMP-2 active isoform was detected in H and H＋F during all periods. Conclusions： Considering the results of the present study, electromagnetic stimulation in association with the A. vera extract promoted an increase in the number of fibroblasts, GAGs content, MMP-2 activity, the deposition and organization of collagen fibers, favoring the repair of injuries to second degree burns, and may also present therapeutic potential in this injury type.