Molecular basis for identification of species/isolates of gastrointestinal nematode parasites

作     者：Ahmed M Singh MN Bera AK Bandyopadhyay S Bhattacharya D 

作者机构：Eastern Regional Station of Indian veterinary Research Institute37-Belgachia Roadkolkata-37West BengalIndia 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2011年第4卷第8期

页      面：589-593页

核心收录：

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：the Director of Indian Veterinary Research Institute(Deemed University) Bareilly  Izatnagar UP for providing financial support to conduct the research work 

主　　题：Gastrointestinal parasites Genomic DNA rDNA mtDNA Species Isolates Genetic diversity 

摘      要：Gastrointestinal（GI） parasitism is the most serious constraint throughout the world in small ruminants which causes significant production loss in *** parasites are major contributor to reduce productivity in terms of meat,milk and wool in *** of GI parasite is done primarily by anthelmintic treatment where choice and schedule of treatment is done after identification and quantitation of individual *** of GI parasites is done through microscopic method by identifying specific morphological characteristics of egg and larva（L3）.Since most of parasite eggs are having similar morphological characteristics, identification up to species level through microscopy is not possible in most of *** address this issue,molecular techniques are the viable alternative for identification of species as well as molecular level differences within a species（isolates） of *** DNA based molecular techniques ***,AFLP,RAPD,RFLP,PCR-SSCP,real time PCR,DNA microarray *** been used for identification and to assess the genetic diversity among parasite *** identification of species,the characteristic sequence of genomic DNA of different species should differ to allow the delineation of species,but at the same time,no/minor variation within the species should *** contrast,for purpose of identifying population variants（strains/isolates）, a considerable degree of variation in the sequence should exist within a *** target regions,including nuclear ribosomal DNA（rDNA）,mitochondrial DNA（mtDNA） or repetitive DNA elements（microsatellite loci）,which show considerable variation in the number of repeats within individuals have been employed to achieve the identification of parasites species or strain.