Effect of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide concentrations on the mechanical and biological characteristics of cross-linked collagen fibres for tendon repair

作     者：Zafar Ahmad Jennifer H.Shepherd David V.Shepherd Siddhartha Ghose Simon J.Kew Ruth E.Cameron Serena M.Best Roger A.Brooks John Wardale Neil Rushton 

作者机构：Orthopaedic Research UnitDepartment of Surgery University of CambridgeAddenbrooke’s HospitalCambridgeCB20QQUK Department of Materials Science and MetallurgyUniversity of CambridgeCambridgeCB30FSUK Tigenix LtdCambridgeCB40FYUK 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2015年第2卷第2期

页      面：77-85页

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：Seventh Framework Programme FP7: 320598 

主　　题：biopolymer scaffolds soft tissue 

摘      要：Reconstituted type I collagen fibres have received considerable interest as tendon implant materials due to their chemical and structural similarity to the native *** produced through a semi-continuous extrusion process were cross-linked with different concentrations of the zerolength cross-linker 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC)in combination with N-hydroxysuccinimide(NHS).Tensile properties of the fibres were considered,along with imaging of both surface structure and fibrillar *** of the fibres to bacterial collagenase was investigated and fibre sections seeded with human tendon cells for biological characterization,including cell adhesion and *** work clearly demonstrated that whilst the concentration of EDC and NHS had no significant effect on the mechanics,a higher concentration was associated with higher collagenase resistance,but also provided a less attractive surface for cell adhesion and proliferation.A lower cross-linking concentration offered a more biocompatible material without reduction in mechanics and with a potentially more optimal degradability.