Effects of special brain area regional cerebral blood flow abnormal perfusion on learning and memory function and its molecular mechanism in rats

作     者：Lingbin KONG Zhiyin YANG Rui AN Shouhua DING 

作者机构：Jining Medical CollegeShandong Institute of Behavioral Medical ScienceJining 272000China Department of Nuclear MedicineUnion HospitalTongji Medical College of Huazhong University of Science and TechnologyWuhan 430030China 

出 版 物：《Frontiers in Biology》 (生物学前沿（英文版）)

年 卷 期：2008年第3卷第2期

页      面：147-153页

核心收录：

学科分类：1002[医学-临床医学] 10[医学] 

基　　金：supported by the Foundation of Science and Technology Bureau (No.2004JH006) Scientific Research of Shandong Province,China (No.JOTYE12) 

主　　题：rCBF learning memory function correlation frontal lobe hippocampus molecular mechanism 

摘      要：To study the effect of special brain area regional cerebral blood flow(rCBF)abnormal perfusion on learning and memory function and its molecular mechanism,64 adult male healthy Sprague-Dawley(SD)rats were randomly divided into two groups,the false operation group(control group)and the operation group(model group).After surgical operation,the operation group undertook bilateral common carotid artery permanent ligation,while the other group did *** and memory function were measured by Y-maze at 4 h,8 h,24 h and 3 d after surgical operation,*** rCBF of the right frontal lobe and hippocampus was also detected by the PerifluxPF model laser Doppler flowmetry,and the expressions of c-fos or c-jun or Bcl-2 and Bax were also measured by immune histochemistry S-P method *** showed that the rCBF of the right frontal lobe and hippocampus in the operation group was significantly lower than that in the false operation group(P,0.05).The learning indexes,error number(EN),day of reach standard and total reaction time(TRT)in the operation group,were significantly higher than that in the false operation group(P,0.05).However,the initiative evasion rate in the operation group was significantly lower than that in the false operation *** study also found that the rCBF was relatively more,the indexes(EN,the day of reach standard and TRT)relatively fewer,but the initiative evasion rate and the memory keeping rate were relatively *** positive expression and the average absorbency of Fos and Jun in the operation group were significantly higher than that in the false operation group(P0.05).Furthermore,Bax and Bcl-2 positive cells were all increased over time in the operation group,and the expression ratio of Bax/Bcl-2 in the operation group was significantly higher than that in the false operation group(P0.01).In conclusion,rCBF decrease can impair the learning and memory function in rats,which may be related to the increase of the expression