Identification of superoxide dismutase isoenzymes in tobacco pollen

作     者：Jing WANG Xuequn LIU Guanghui YU 

作者机构：Key Laboratory for Biotechnology of the State Ethnic Affairs CommissionCollege of Life ScienceSouth-Central University for NationalitiesWuhan 430074China 

出 版 物：《Frontiers in Biology》 (生物学前沿（英文版）)

年 卷 期：2009年第4卷第4期

页      面：442-445页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：This work was supported by the Natural Science Foundation of South-Central University for Nationalities(No.yzz07001) sponsored by the National Natural Science Foundation of China(Grant No.30700427) supported by the Academic lnnovation Team for Plant Development and Genetics in South-Central University for Nationalities(XQ LIU group) 

主　　题：tobacco pollen reactive oxygen species superoxide dismutase fertilization 

摘      要：We investigated the possible existence of superoxide dismutase(SOD;EC 1.***.1.1)isoenzymes in the pollen of Nicotiana tabacum(Petit Havana SR-1 cultivar).To detect SOD activity,crude extracts from tobacco pollen were subjected to native polyacrylamide gel electrophoresis followed by staining with nitroblue tetra-zolium(NBT).The presence of six SOD isoenzymes was detected in tobacco *** with SOD inhibitors indicated the presence of one manganese SOD(Mn SOD),five copper-zinc SOD(Cu/Zn SOD)isoenzymes,and the absence of iron SOD(Fe SOD).