Intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease

作     者：Kriszta Molnár dám Vannay Beáta Szebeni Nóra Fanni Bánki Erna Sziksz ron Cseh Hajnalka Gyrffy Péter László Lakatos Mária Papp András Arató Gábor Veres 

作者机构：First Department of PediatricsSemmelweis UniversityH-1083 BudapestHungary Research Group for Pediatrics and NephrologySemmelweis University and Hungarian Academy of SciencesH-1083 BudapestHungary SE-MTA "Lendulet" Diabetes Research GroupH-1083 BudapestHungary Second Department of PathologySemmelweis UniversityH-1091 BudapestHungary First Department of MedicineSemmelweis UniversityH-1083 BudapestHungary Second Department of Medicine University of Debrecen H-4032 Debrecen Hungary 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2012年第18卷第25期

页      面：3254-3259页

核心收录：

学科分类：1002[医学-临床医学] 100202[医学-儿科学] 10[医学] 

基　　金：Supported by Grants OTKA-76316,OTKA-K81117,and ETT-028-02 (Veres G and Vannay á are holders of the János Bolyai Research grant) János Bolyai Research Scholarship of the Hungarian Academy of Sciences 

主　　题：碱性磷酸酶 肠道疾病 肠黏膜 儿童 溃疡性结肠炎 免疫荧光染色 蛋白水平 Toll样受体 

摘      要：AIM:To investigate intestinal alkaline phosphatase(iAP) in the intestinal mucosa of children with inflammatory bowel disease(IBD).METHODS:Colonic biopsy samples were taken from 15 newly diagnosed IBD patients and from 10 healthy *** IBD patients,specimens were obtainedboth from inflamed and non-inflamed *** iAP mRNA and protein expression was determined by reverse transcription-polymerase chain reaction and Western blotting analysis,*** localization of iAP and Toll-like receptor(TLR) 4 was investigated by immunofluorescent ***:The iAP protein level in the inflamed mucosa of children with Crohn s disease(CD) and ulcerative colitis(UC) was significantly decreased when compared with controls(both P 0.05).Similarly,we found a significantly decreased level of iAP protein in the inflamed mucosa in CD compared with non-inflamed mucosa in CD(P 0.05).In addition,the iAP protein level in inflamed colonic mucosa in patients with UC was decreased compared with non-inflamed mucosa in patients with CD(P 0.05).iAP protein levels in the non-inflamed mucosa of patients with CD were similar to *** mRNA expression in inflamed colonic mucosa of children with CD and UC was not significantly different from that in non-inflamed colonic mucosa with *** of iAP mRNA in patients with noninflamed mucosa and in controls were ***-localization of iAP with TLR4 showed intense staining with a dotted-like *** was present in the inflamed and non-inflamed mucosa of patients with CD,UC,and in control biopsy specimens,irrespective of whether it was present in the terminal ileum or in the ***,the fluorescent signal of TLR4 was more pronounced in the colon compared with the terminal ileum in all groups ***:Lower than normal iAP protein levels in inflamed mucosa of IBD patients may indicate a role for iAP in inflammatory lesions in *** on our results,administration of exogenous iAP enzyme to pa