Evaluation of aqueous and ethanol extract of bioactive medicinal plant,Cassia didymobotrya(Fresenius)Irwin & Barneby against immature stages of filarial vector,Culex quinquefasciatus Say(Diptera:Culicidae)

作     者：Raja Nagappan 

作者机构：Department of BiologyFaculty of Natural and Computational SciencesPost Box 196University of Gondar 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2012年第2卷第9期

页      面：707-711页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by University of Gondar(UOG/Budget/no.6215) 

主　　题：Cassia didymobotrya Culex quinquefasciatus Larvicide Pupicide Aqueous Ethanol Extracts 

摘      要：Objective:To evaluate aqueous and ethanol extract of Cassia didymobotrya leaves against immature stages of Culex ***:The mortality rate of immature mosquitoes was tested in wide and narrow range concentration of the plant extract based on WHO standard *** wide range concentration tested in the present study was 10 000,1 000,100,10 and1 mg/L and narrow range concentration was 50,100,150,200 and 250 mg/***:2nd instar larvae exposed to 100 mg/L and above concentration of ethanol extract showed 100%*** stages such as 3rd,4th and pupa,100%mortality was observed at 1 000 mg/L and above concentration after 24 h exposure *** aqueous extract all the stages 100%mortality was recorded at 1000 mg/L and above *** narrow range concentration 2nd instar larvae 100%mortality was observed at 150 mg/L and above concentration of ethanol *** remaining stages 100%mortality was recorded at 250 mg/*** aqueous extract all the tested immature stages 100%mortality was observed at 250 mg/L concentration after 24 h exposure *** results clearly indicate that the rale of mortality was based dose of the plant extract and stage of the ***:From this study it is confirmed and concluded that Cassia didymobotrya is having active principle which is responsible for controlling Culex *** isolation of biouctive molecules and development of simple formulation technique is important for large scale implementation.