Establishment of the model of white blood cell membrane chromatography and screening of antagonizing TLR_4 receptor component from Atractylodes macrocephala Koidz

作     者：LI Cuiqin HE Langchong 

作者机构：School of Medicine Xi'an Jiaotong University Xi'an 710061 China School of Medicine Xi'an Jiaotong University Xi'an 710061 China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2006年第49卷第2期

页      面：182-189页

核心收录：

学科分类：07[理学] 08[工学] 

主　　题：white blood cell membrane chromatography, TLR4 receptor, atractylenolide I, anti-inflammatory effect. 

摘      要：A model of white blood cell membrane chromatography (WB-CMC) was established to screen active component from Atractylodes macrocephala Koidz. The component can antagonize Toll-like receptor 4 (TLR4); inhibit inflammatory reaction. In the model of WB-CMC, cell membrane stationary phase (CMSP) was prepared by immobilizing the rabbit white blood cell membrane (WBCM) onto the surface of silica carrier; taxinol was used as a model molecule. The active component which can act on WBCM; its receptor (such as TLR4) as an effective target in A. macrocephala was determined by using a replacement experiment. The anti-inflammatory effects of the active component were tested by using pharmacological methods in vivo. The results indicated that the retention characteristics of atractylenolide I as active component was similar to that of taxinol in the model of WB-CMC. And so, atractylenolide I acted on the WBCM; TLR4; its anti-inflammatory activity was related with antagonizing TLR4. Therefore, the interaction between the active component; WBCM; its receptor can be simulated by the model of WB-CMC in vitro. This model can be used to screen active components; to study effective characteristics for acting on definite targets.