Diagnostic application of serum proteomic patterns in early gastric cancer patients by SELDI-TOF-MS

作     者：Chunwei Li Dianliang Zhang Jian Zhang Fengbo Sun Lei Mi 

作者机构：Department of General Surgery Affiliated Hospital of Medical College Qingdao University Qingdao 266003 China 

出 版 物：《The Chinese-German Journal of Clinical Oncology》 (中德临床肿瘤学杂志（英文版）)

年 卷 期：2011年第10卷第5期

页      面：261-265页

学科分类：090603[农学-临床兽医学] 0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by a grant from the Key Project of Shandong Scientific andTechnology (No. 2008GG30002034) 

主　　题：gastric cancer biomarkers SELDI-TOF-MS serum proteomic patterns 

摘      要：Objective:Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS) is one of the currently used techniques to identify biomarkers for *** study was planned to make a proteomic analysis on the serum of stage I gastric cancer patients and establish a early diagnostic model for identifying stage I gastric cancer ***:A total of 229 serum samples including 169 pathologically confirmed gastric cancer patients(stage I:n = 47;stage II:n = 39;stage III:n = 46;stage IV:n = 37) and 60 gastritis patients were analyzed by SELDI-TOF-MS Protein Chip array *** resulting SELDI-TOF-MS spectral data were analyzed using the Biomarker WizardTM and Biomarker Patterns software to find differential proteins and develop a classification tree for early gastric ***:Twelve proteins were significantly over expressed(P0.05,Student t-test) in patients with gastric cancer compared to patients with *** them,three protein masses with 2873,6121 and 7778 m/z was found to be differentially expressed solely in patients suffering from stage I gastric *** three protein masses construct the decision tree which could effectively distinguish stage I gastric cancer samples from control samples,achieving a sensitivity and specificity of 89.36(42/47) and 97.32%(182/187),***:Serum Proteomic Patterns can be used for the quick diagnosis of stage I gastric ***-TOF-MS is a useful tool for the detection and identification of new protein markers in serum.