Regeneration of Plants from Embryogenic Cell Suspensions of cv.“Datil”(Musa AA):Morphological Evaluation of Plants in the Field
作者机构：Biotechnology LaboratoriesTropical Agricultural Research and Higher Education Center(CATIE)Turrialba 30501Costa Rica National Banana Corporation(CORBANA)Guápiles 390-7210PococíCosta Rica
出 版 物：《Journal of Agricultural Science and Technology(B)》 (农业科学与技术（B）)
年 卷 期：2018年第8卷第1期
页 面：29-41页
学科分类：08[工学] 081201[工学-计算机系统结构] 0812[工学-计算机科学与技术（可授工学、理学学位）]
主 题：Musa Pisang Mas somatic embryogenesis cell suspension plant regeneration field evaluation
摘 要：The full cycle from embryogenic callus(EC)induction to field evaluation of regenerated plants is reported for the first time for banana cv.“Datil(Musa AA).Immature male flowers were used for EC induction on a modified M1 culture *** ideal type of EC was obtained in the presence of 4.5μM and 9.0μM 2,4-dichlorophenoxyacetic acid(2,4-D).Embryogenic cell suspensions were established in both concentrations of 2,4-D;however,only the suspensions from ideal callus(IC)formed with 4.5μM of 2,4-D were regenerated.A histological study revealed the formation of structurally different cell masses during the regeneration phase of cv.“Datil.The embryos germination was characterized by the growth cellular aggregates,indicating the possible occurrence of secondary *** and acclimatization of the plants were carried out in a normal manner as observed for other *** evaluation of the plants’genetic stability was based on observation of morphological *** the vegetative growth phase,6.61%of the plants presented drooping leaves and deformed *** traits,however,did not affect further plant growth during flowering and harvest *** produced fruits were of good *** the present study indicates that this cultivar may be stable genetically.
维普期刊数据库