Microscopy: looking into the mirror

作     者：Xusan Yang Peng Xi 

作者机构：Department of Biomedical EngineeringCollege of EngineeringPeking UniversityBeijingChina 

出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)

年 卷 期：2018年第7卷第1期

页      面：989-991页

核心收录：

学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学] 

主　　题：mirror excitation mentioned 

摘      要：Mirrors can create a virtual excitation source for optical microscopy,which can greatly enhance the spatiotemporal resolution of different fluorescence microscopy techniques,thus advancing toward longterm live cell *** recent decades,many new discoveries have been obtained using novel optical microscopic techniques,such as confocal,multiphoton,super resolution,and light sheet microscopies,which have attracted intensive interest from biologists working in various ***,advances in live cell fluorescence microscopy are facing multiple challenges,such as low resolution,poor signal-to-background ratio(SBR),insufficient imaging speed,and ***,these grand challenges share a common solution:*** placing a reflective mirror after an objective,the beam can be reflected,and a“virtualexcitation source can be generated without additional *** conceptually simple approach provides an easy solution to the abovementioned challenges,such as greater signal,better contrast,improved optical section ability at relative low cost,and facilitating live cell imaging with improved spatial resolution at a high speed.