Toxicity Assessment of Cadinene Sesquiterpenes from Eupatorium adenophorum in Mice

作     者：Can-Bin Ouyang Xiao-Man Liu Qi Liu Jie Bai Hou-Yong Li Yuan Li Qiu-Xia Wang Dong-Dong Yan Lian-Gang Mao Aocheng Cao Mei-Xia Guo 

作者机构：Department of PesticidesInstitute of Plant ProtectionChinese Academy of Agricultural SciencesMinistry of Agricultureand State Key Laboratory for Biology of Plant Diseases and Insect PestsBeijing 100193People’s Republic of China National Engineering Laboratory for Crop Efficient Water Use and Disaster Mitigationand Key Laboratory of Dryland AgricultureMinistry of AgricultureInstitute of Environment and Sustainable Development in AgricultureChinese Academy of Agricultural SciencesBeijing 100081People’s Republic of China 

出 版 物：《Natural Products and Bioprospecting》 (应用天然产物（英文）)

年 卷 期：2015年第5卷第1期

页      面：29-36页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Special Nonprofit Agricultural Industrial Research Fund(20080321 201103027)of China 

主　　题：Eupatorium adenophorum Sesquiterpenes Histopathology Environmental toxicity Biopesticide 

摘      要：This study evaluated toxic efficacy of Eupatorium adenophorum extracts,against the Kunming *** acute study,we firstly tested median lethal dose(LD50)in mice of three cadinene sesquiterpenes 2-deoxo-2-(acetyloxy)-9-oxoageraphorone(DAOA),9-oxo-agerophorone(OA)and 9-oxo-10,11-dehydro-agerophorone(ODA)from Eupatorium adenophorum(Ea).DAOA(215–4640 mg/kg BW,given orally)showed lowest LD50 at 926 mg/kg BW for male mice in contrast with OA(1470 mg/kg BW)and ODA(1470 mg/kg BW).In sub-acute study,repeated doses(75–300 mg/kg BW,for 7 days)of DAOA/OA increased blood parameters,liver and spleen index in dose dependent relationship,along with decrease in thymus *** blood biochemical and histopathological examination showed that DAOA/OA dose 300 mg/kg BW significantly causes pathological changes of hepatic lobules and hepatocytes,which are consistent with cholestasis and hepatic injury.75 mg/kg dose of DAOA/OA was found to be approximately/totally safe over the span of 7 days treatment showing no change in all above described *** sesquiterpenes guarantee low risk to environment as a type of low toxic botanical components,which may find potential application in biopesticides development field.