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Quantification of total phenol,flavonoid content and pharmacognostical evaluation including HPTLC fingerprinting for the standardization of Piper nigrum Linn fruits

Quantification of total phenol,flavonoid content and pharmacognostical evaluation including HPTLC fingerprinting for the standardization of Piper nigrum Linn fruits

作     者:Aftab Ahmad Asif Husain Mohd Mujeeb Shah Alam Khan Hani Abdullah Anber Alhadrami Anil Bhandari 

作者机构:Health Information Technology DepartmentJeddah Community CollegeKing Abdulaziz University Faculty of Pharmaceutical SciencesJodhpur National University Department of Pharmaceutical ChemistryFaculty of PharmacyJamia Hamdard.New DelhiIndia Department of PharmacognosyFaculty of PharmacyJamia HamdardNew Delhi.India Department of PharmacyOman Medical College Department of Medical Laboratory TechnologyFaculty of Applied Medical ScienceKing Abdulaziz University 

出 版 物:《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志(英文版))

年 卷 期:2015年第5卷第2期

页      面:101-107页

学科分类:0710[理学-生物学] 0831[工学-生物医学工程(可授工学、理学、医学学位)] 1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基  金:Supported by AYUSH.Ministry of Health and Family Welfare.Government of India[Grant No.CCRUM-UPC-Ⅱ(3-15/2009.CCRUM/UPC)] 

主  题:Piper nigrum L.fruits Piperaceae HPTLC fingerprint Black pepper 

摘      要:Objective:To carry out the physicochemical and phytochemical standardization with high performance thin layer chromatography fingerprinting of Piper nigrum L.(***)fruits in order to ascertain the standard pharmacognostical parameters of this king of ***:Many standardization parameters like extractive values,total ash value,water soluble ash value and acid insoluble ash,moisture content,loss on drying and pH values of *** *** were *** method of Harborne was adopted for the preliminary phytochemicals *** of total phenolic and flavonoid contents,pesticides residues,aflatoxin and heavy metals were also ***-high performance thin layer chromatography system was used for fingerprinting of methanolic extract of *** ***:The results of phytochemicals testing indicated the presence of carbohydrates,phenolic compounds,flavonoids,alkaloids,proteins,saponins,lipids,sterols and tannins in various solvent *** phenolic and flavonoid contents in methanolic extract were found to be 1.728 1 mg/g and 1.087 ug/g,*** metals concentrations were found to be within standard *** and pesticides residues were ***:The outcome of this study might prove beneficial in herbal industries for identification,purification and standardization of *** ***.

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