Exocytosis determination of SH-SY5Y single cell stimulated by diff erent stimulants on indium tin oxide (ITO) micro-pore electrode
作者机构：Department of ChemistryEast China Normal UniversityShanghaiChina Department of ChemistryZhoukou Normal UniversityZhoukouChina
出 版 物：《BIOCELL》 (生物细胞（英文）)
年 卷 期：2013年第37卷第3期
页 面：77-83页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
基 金：the National Nature Science Foundation of China(Grant No.21075042)
主 题：amperometric monitoring microelectrodes mechanism response time
摘 要：The human neuroblastoma SH-SY5Y cell line has been used as a model to study mechanisms of neurotransmitter *** order to study the mechanism of SH-SY5Y single cell exocytosis stimulated by different stimulants,including high K+,3-(1-nitroso-2-pyrrolidinyl)pyridine and nicotine,a type of indium tin oxide(ITO)micro-pore electrode was used to obtain the corresponding amperometric response *** the cell is stimulated by 0.1 M K+,almost immediate exocytosis could be detected,due to the rapid depolarization of cell ***,the stimulations with 1 mM nicotine and 3-(1-nitroso-2-pyrrolidinyl)pyridine result in a short delay between stimulation and exocytosis,which can be correlated with the time needed for binding of the stimulant to the nicotinic acetylcholine(ACh)receptor and the induction of post-binding ***,the response time of SH-SY5Y single cell exocytosis is significantly affected by the exocytosis mechanisms.
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