Syntheses of bifunctional molecules containing [12]aneN_3 and carbazol moieties as effective DNA condensation agents

作     者：YAN Hao YUE Pan LI ZhiFen GUO ZhiFo LU ZhongLin 

作者机构：Key Laboratory of Theoretical and Computational PhotochemistryMinistry of Education College of ChemistryBeijing Normal University 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2014年第57卷第2期

页      面：296-306页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：supported by the Program for New Century Excellent Talents in Universities(NCET-08-0054) the National Natural Science Foundation of China(20972019) 

主　　题：DNA condensation macrocyclic polyamines carbazoles triazole gel electrophoresis AFM 

摘      要：Bifunctional molecules containing macrocyclic polyamine [12]aneN3 and carbazol units, 1-4, have been efficiently synthe sized and fully characterized. Through gel electrophoresis, atomic force microscopy, and dynamic light scattering experiments, compounds 3 and 4b bearing both [12]aneN3 and carbazol moieties showed effective DNA condensation ability at the concen- tration of 80 jaM. Investigations from EB displacement fluorescence spectra, viscosity titration, and ionic strength effects re vealed that the effective DNA condensation comes from the appropriate combination of carbazol and [12]aneN3 units in the bifunctional molecules, and the DNA condensation process is reversible. The incorporation of triazole units in the molecules clearly reduced the cytotoxicity.