Optimization of fermentation medium for xylanase-producing strain Xw2
Optimization of fermentation medium for xylanase-producing strain Xw2作者机构：College of Life Sciences Fujian Normal University Fuzhou 350108 China Key Laboratory of Developmental and Neural Fuzhou 350108 China Key Laboratory of Sugarcane Biology and Genetic Breeding of Ministry of Agriculture Fuzhou 350108 China Environmental Protection Agency Fuzhou Sanming 350400 China
出 版 物：《Frontiers in Biology》 (生物学前沿（英文版）)
年 卷 期：2013年第8卷第6期
页 面：611-617页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 081703[工学-生物化工] 08[工学] 0817[工学-化学工程与技术] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 10[医学]
基 金：supported by grants from the Key Laboratory of Sugarcane Biology and Genetic Breeding of Ministry of Agriculture the foundation of the Construction of Modern Agricultural Technology System of China
主 题：enzyme activity fermentation Plackett-Burman test xylan
摘 要：To improve the fermentation yield of xylanase by optimizing the fermentation conditions for strain Xw2, a Plackett-Burman design was used to evaluate the effects of eight variables on xylanase production by strain Xw2. The steepest ascent （descent） method was used to approach the optimal response surface experimental area. The optimal fermentation conditions were obtained by central composite design and response surface analysis. The results showed that the composition of the optimal fermentation medium was corn cob ＋ 1.5% wheat bran （1：1）, 0.04% MnSO4, 0.04% K2HPO4. 3H2O, and an inoculum size of 6% in 50 mL liquid volume （pH = 6.0）. The optimal culture conditions were 28oc at 150 r/min for 54.23 h. The results of this study can serve as the basis for the industrial production and application of xylanase.
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