Genetic analysis and fine mapping of the pubescence gene GL6 in rice (Oryza sativa L.)

作     者：ZENG YueHui ZHU YongSheng LIAN Ling XIE HongGuang ZHANG JianFu XIE HuaAn 

作者机构：Rice Research Institute Fujian Academy of Agricultural Sciences Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology/National Engineering Laboratory of Rice/South Base of National Key Laboratory of Hybrid Rice of China 

出 版 物：《Chinese Science Bulletin》 (Chinese Science Bulletin)

年 卷 期：2013年第58卷第24期

页      面：2992-2999页

核心收录：

学科分类：080202[工学-机械电子工程] 08[工学] 09[农学] 0901[农学-作物学] 0802[工学-机械工程] 

基　　金：supported by the National Key Basic Research Program of China (2012CB723003) National High Technology Research and Development Program of China (2010AA101801 and 2010AA101804) Science and Technology Innovation Foundation of Fujian Academy of Agricultural Sciences supported by the Financial Department of Fujian Government (STIF-Y04) 

主　　题：水稻基因 精细定位 遗传分析 简单重复序列 籼稻品种 第6号染色体 F2群体 叶片表面 

摘      要：The pubescence of the leaf blade surface is an important agronomic characteristic for rice morphology and significantly influences rice growth as well as physiological characteristics. This characteristic was analyzed in F1 and F2 plants derived by crossing cultivar 75-1-127 with the indica cultivar Minghui 63, as well as the glabrous cultivar Lemont and indica cultivar 9311. Results indicated that the pubescence of the leaf blade surface was a dominant trait and controlled by a single gene. The GL6 gene was primarily mapped on rice chromosome 6 with recessive F2 population derived from 75-1-127/Minghui 63 by combining bulked segregation analysis and recessive class analysis using the Mapmaker3.0/MapDraw software. The genetic distances between the simple sequence repeat markers RM20491 and RM20547 were 7.2 and 2.2 cM, respectively. The GL6 gene was fine mapped in the interval between InDel-106 and InDel-115 at genetic distances of 0.3 and 0.1 cM, respectively. The large, recessive F2 population was derived from 75-1-127/Minghui 63. A high-resolution genetic and physical map of GL6 was constructed. Derived from the map-based sequences published by the International Rice Genome Sequencing Project, the GL6 gene was localized at an interval of 79 (japonica) and 116.82 kb (9311) bracketed by InDel-106 and InDel-115 within the BAC accession numbers AP008403 and AP005760. Seven annotated genes (japonica) and eight annotated genes (9311) were present. The basis was further set for GL6 cloning and function analysis.