The real-time second-derivative spectral analysis of C-terminus of engineering product by RP-HPLC

作     者：张丽华 屠红旻 邵晓霞 吴高德 徐来根 夏其昌 

作者机构：Shanghai Institute of Biochemistry Chinese Academy of Sciences Shanghai 200031 China 

出 版 物：《Chinese Science Bulletin》 (科学通报(英文版))

年 卷 期：1995年第40卷第23期

页      面：1997-2001页

核心收录：

学科分类：0710[理学-生物学] 071011[理学-生物物理学] 07[理学] 

基　　金：Project supported by 863. 

主　　题：second-derivative spectrum C-terminal analysis new-type IL-2 (Ser-125) hEGF. 

摘      要：N-terminal and C-terminal analysis of genetic engineering recombinant proteins are two criteria of determining their primary structure. The N-terminal sequencing relies heavily upon the Edman degradation method, which has been automated with commercially available sequencers. The maximum number of residues which may be placed in sequence in a single run is about 50. In the present decade, improvements in this technique made it