Mapping of Hd-6-2 for Heading Date Using Two Secondary Segregation Populations in Rice

作     者：ZHANG Hua LIU Xu YANG Yongyi XUAN Ning YAO Fangyin 

作者机构：Bio-Tech Research Center Shandong Academy of Agricultural Sciences 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2018年第25卷第3期

页      面：161-168页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 0902[农学-园艺学] 

基　　金：financially supported by the National Key Research and Development Program of China (Grant Nos. 2016YFD0100903-9 and 2016YFD0100101-14) the Natural Science Foundation of Shandong Province (Grant No. ZR2014CQ007) the Rice Industry Technology Program of Shandong Province, China (Grant No. SDAIT-17-03) 

主　　题：rice single segment substitution line heading date gene mapping Hd3a 

摘      要：Heading date is one of the most important traits for rice adaption to different cultivation areas and crop seasons. In this study, two single segment substitution lines(SSSLs), W31-41-61-3-11-3-6-7(W31-SSSL) and W32-59-80-2-11-1-10(W32-SSSL) with substituted intervals derived from the donor parents IR66897 B(W31) and IR66167-27-5-1-6(W32), respectively, with Huajingxian 74(HTX74) were found to comprise a gene for extremely late-heading date, and the gene was tentatively designated as Hd-6-2. Two secondary F2 segregating populations were developed by crossing the two heterozygous SSSLs with HJX74 to map Hd-6-2 gene. According to phenotype analysis of the two mapping populations, the late heading date trait was controlled by a major recessive gene. In the segregation population derived from W31-SSSL, Hd-6-2 was mapped on chromosome 6 between PSM677 and RM204 with the genetic distances of 1.3 and 2.7 c M, respectively. In the population of W32-SSSL, the gene for heading date was mapped to the similar region as Hd-6-2 and co-segregated with PSM672. The sequence alignment of Hd3 a in the coding domains and promoter regions of HJX74 and W31-SSSL are completely consistent, whereas there was a great difference between W32-SSSL and HJX74, suggesting that Hd3 a could hardly be the main cause of the heading date variation in W31-SSSL, but it was probably the main reason for the change of heading stage in W32-SSSL.