Effects of Plasmodium falciparum—infected erythrocytes on matrix metalloproteinase-9 regulation in human microvascular endothelial cells

作     者：Sarah D' Alessandro Nicoletta Basilico Mauro Prato 

作者机构：Dipartimento di Sanita Pubblica-Microbiologia-VirologiaUniversita di MilanoMilanoItaly Dipartimento di GeneticaBiologia e BiochimicaFacolta di Medicina e ChirurgiaUniversita di TorinoTorinoItaly 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2013年第6卷第3期

页      面：195-199页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by Universita di Milano(PUR.2009) to Nicoletta Basilico and Charity Funds from Mrs.Franca Squazza to Mauro Prato Mauro Prato holds a professorshipgranted by Universita Torino and Azienda Sanitaria Locale-19(ASL-19) 

主　　题：Plasmodium falciparum Malaria Endothelial cells Matrix metalloproteinases Tissue inhibitors of metalloproteinases Gelatinases 

摘      要：Objective:To investigate the regulation of matrix metalloproteinases(MMPs) and tissue inhibitors of melalloproleinases(TIMPs) in human microvascular endothelium(HMEC-1) exposed to erythrocytes infected by different strains of Plasmodium falciparum(***).Methods: HMEC—1 eells were co—incubated for 72 h with erythrocytes infected by late stage trophozoite of D10(chloroquine-sensilive) or W 2(chloroquine-resistant) *** *** supernatants were then collected and the levels of pro- or active gelatinases MMP-9 and MMP-2 were evaluated by gelatin zymograpln and *** release of pro-MMP-9,***-1 and TIMP-1 proteins was analyzed by western blotting and ***:Infected erythrocytes induced de novo proMMP-9 and MMP-9 *** basal levels of proMMP-2 were altered,nor active MMP-2 was ***-3 and MMP-1 secretion was significant!) enhanced,whereas basal TIMP-1 was *** effects were similar for both strains. Conclusions:*** parasites,either chloroquine-sonsitive or -resistant,induce the release of active MMP-9 protein from human microvascular endothelium,by impairing balances between proMMP-9 and its inhibitor,and by enhancing the levels of its *** work provides new evidence on MMP involvement in malaria,pointing at MMP-9 as a possible target in adjuvant therapy.