Protein-mimicking nanoparticle(Protmin)-based nanosensor for intracellular analysis of metal ions

作     者：Dan Zhu Dong-Xia Zhao Jia-Xuan Huang Jiang Li Xiao-Lei Zuo Li-Hua Wang Chun-Hai Fan 

作者机构：Division of Physical Biology and Bioimaging CenterShanghai Synchrotron Radiation Facility Shanghai Institute of Applied Physics Chinese Academy of Sciences Key Laboratory for Organic Electronics and Information Displays Institute of Advanced Materials (IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Jiangsu Key Laboratory for BiosensorNanjing University of Posts and Telecommunications 

出 版 物：《Nuclear Science and Techniques》 (核技术（英文）)

年 卷 期：2018年第29卷第1期

页      面：82-89页

核心收录：

学科分类：08[工学] 0827[工学-核科学与技术] 

基　　金：supported by the National Natural Science Foundation of China(Nos.21390414 and 21605087) the Chinese Academy of Sciences(No.QYZDJ-SSW-SLH031) the China Postdoctoral Science Foundation funded project(No.BX201700123) the Scientific Research Foundation of Nanjing University of Posts and Telecommunications(No.NY215058) the Natural Science Fund for Colleges and Universities in Jiangsu Province(16KJB150032) 

主　　题：Protmin Nanosensor Poly-adenine Lead ion Intracellular detection 

摘      要：In this study, we designed and applied proteinmimicking nanoparticles(Protmin) as an intracellular nanosensor for in vivo detection of lead ions(Pb^(2+)).Monodispersed gold nanoparticles(Au NPs) of 13 nm in diameter were modified using poly-adenine-tailed Pb^(2+)-specific 8–17 DNAzyme to form a spherical and functional Protmin. Substrate strands modified with a fluorophore at the 50 end and a quencher at the 30 end were bound to DNAzyme. Pb^(2+) facilitated cleavage of DNAzyme to release the fluorophore-modified short strands to generate fluorescence. We observed rapid kinetics of the Protmin nanosensor, for which the typical assay time was 10 ***, we demonstrated the Protmin nanosensor could readily enter living cells and respond to Pb^(2+) in the intracellular environment. The broad of range of Protmindesigns will be useful for advancing biological and medical applications.