Efficient and Soluble Expression of α Protein of Clostridium perfringens and Preparation of Genetic Engineering Subunit Vaccine
Efficient and Soluble Expression of α Protein of Clostridium perfringens and Preparation of Genetic Engineering Subunit Vaccine作者机构：China Animal Disease Control Center Beijing 102600 China
出 版 物：《Animal Husbandry and Feed Science》 (动物与饲料科学（英文版）)
年 卷 期：2017年第9卷第5期
页 面：284-288,305页
学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学]
基 金：Supported by the 13th Five-Year National Key Research and Development Program(2016YFD0500901)
主 题：Clostridium perfringens α protein Soluble expression and purification Genetic engineering subunit vaccine
摘 要：[Objective] The paper was to develop genetic engineering vaccine that can express α exotoxin antigen protein efficiently without destroying its immunogenicity for preventing and controlling the diseases caused by Clostridium perfringens. [Method] Efficiently expressed soluble recombinant α protein was obtained from Escherichia coli expression system by optimizing codon,removing signal peptide,selecting sequences with better hydrophilicity and antigenicity,and optimizing expression conditions. [Result] Mice obtained higher serum antibody level when immunized by α protein,and the immune protection rates against type A,type B,type C and type D C. perfringens were 100%,90%,85% and 90%,respectively. The antibody titer of mice within 7-14 d after the third immunization reached the peak. [Conclusion]The α protein has good immunogenicity,and can be further used to develop genetic engineering subunit vaccines for preventing C. perfringens.
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