Desiccation tolerance in bryophytes： the rehydration proteomes of Bryum argenteum provide insights into the resuscitation mechanism

作     者：GAO Bei ZHANG Daoyuan LI Xiaoshuang YANG Honglan LIANG Yuqing CHEN Moxian ZHANG Yuanming ZHANG Jianhua Andrew WOOD GAO Bei1＇2, ZHANG Daoyuan1, LI Xiaoshuang1, YANG Honglan1, LIANG Yuqing1, CHEN Moxian2, ZHANG Yuanming1, ZHANG Jianhua2＇3, Andrew WOOD4

作者机构：Key Laboratory of Biogeography and Bioresource in Arid LandXinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqi 830011China School of Life Sciences and State Key Laboratory of AgrobiotechnologyThe Chinese University of Hong KongHong KongChina Department of BiologyFaculty of ScienceHong Kong Baptist UniversityHong KongChina Department of Plant BiologySouthern Illinois University-CarbondaleCarbondaleIL 62901-6509USA 

出 版 物：《Journal of Arid Land》 (干旱区科学（英文版）)

年 卷 期：2018年第10卷第1期

页      面：152-167页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 

基　　金：supported by the Scientific Service Project of Chinese Academy of Sciences (TSS-2015-014-FW-4-3) the National Science Foundation of China-Xinjiang Talent Youth Project (U1403302) 

主　　题：desiccation tolerance B*Tum Physcomitrellapatens proteome iTRAQ rehydration 

摘      要：Bryum argenteum Hedw. is a desiccation tolerant bryophyte and belongs to one of the most important components of the biological soil crusts （BSCs） found in the deserts of Central Asia. Limited information is available on rehydration-responsive proteins in desiccation tolerant plants. As a complement to our previous research analyzing the rehydration transcriptome, we present a parallel quantitative proteomic effort to study rehydration-responsive proteins. Bryophyte gametophores were desiccated （Dry） and rehydrated for 2 h （R2） and 24 h （R24）. Proteins from Dry, R2 and R24 gametophores were labeled by isobaric tags for relative and absolute quantitation （iTRAQ） to determine the relative abundance of rehydration-responsive proteins. A total of 5503 non-redundant protein sequences were identified and 4772 （86.7%） protein sequences were annotated using Gene Ontology （GO） terms and Pfam classifications. Upon rehydration 239 proteins were elevated and 461 proteins were reduced as compared to the desiccated protein sample. Differentially up-regulated proteins were classified into a number of categories including reactive oxygen species scavenging enzymes, detoxifying enzymes, Late Embryogenesis Abundant （LEA） proteins, heat shock proteins, proteasome components and proteases, and photosynthesis and translation related proteins. Furthermore, the results of the correlation between transcriptome and proteome revealed the discordant changes in the expression between protein and mRNA.