Effect of moxibustion on the expression levels of proteins of neuron and neuropeptide in the intestinal tract of rats with Crohn's disease

作     者：QI Qin WU Lu-yi WU Huan-gan LIU Hui-rong WANG Yuan-yuan LIU Ya-nan WANG Cun HUANG Ru-de WANG Xiao-mei 祁琴;吴璐一;吴焕淦;刘慧荣;王圆圆;刘雅楠;王村;黄儒德;王晓梅

作者机构：Shanghai University of Traditional Chinese Medicine Shanghai Research Institute of Acupuncture and Meridia 

出 版 物：《World Journal of Acupuncture-Moxibustion》 (世界针灸杂志（英文版）)

年 卷 期：2017年第27卷第3期

页      面：40-48,62页

核心收录：

学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：Supported by:Youth Scientific Research Project of Shanghai Municipal Commission of Health and Family Planning:2013Q042 National Key Basic Research Development Program(973 Program):2015CB554500 Support Plan of New Century Excellent Talents of Ministry of Education:NCET-13-0907 National Natural Science Foundation:81574079 Shanghai Municipal Youth Science and Technology Venus Project:16QA1403400 

主　　题：moxibustion Crohn's disease S-100 substance P NK1R neuropeptide Y NPY1R NPY2R 

摘      要：Objective To explore the regulating effect of moxibustion on the enteric nervous system of rats with Crohn＇s disease. Methods Ten SD rats were selected randomly from 40 rats as normal control（group A）, and the other 30 rats were established into Crohn＇s disease rat models by adopting clysis method with TNBS. On the basis of modeling successfully, the model rats were randomly divided into model group（group B）, herbs-partitioned moxibustion group（group C） and mild moxibustion group（group D） with 8 rats in each group（4 rats were dead during modeling. After modeling, 2 rats were selected from group A, and 2 rats were selected from models for determination,at last, 8 rats were included in each group）. In group C and group D, herbs-partitioned moxibustion or mild moxibustion was applied on ＂Tiānshū（天枢 ST 25）＂ bilaterally, and the rats in group A and group B were fixed as in treatment groups. HE stain was conducted, and morphological observation was performed on the colonic tissue of rats; the expression levels of proteins of S-100, SP, NPY and their receptors were observed by adopting immunohistochemical method. Results Compared with group A, the expression levels of proteins of S-100, SP and its receptor NK1R, NPY and its receptors NPY1R and NPY2R in the intestinal tract of rats in model groups increased obviously, and the differences were statistically significant.（P_（S-100）0.01, P_（SP）0.05, P_（NK1R）0.01, P_（NPY）0.05, P_（NPY1R）0.05, P_（NPY2R）0.01）, after treatment with drug-paste interposed moxibustion and mild moxibustion, the levels reduced significantly（P_（S-100）0.05, P_（SP）0.05, P_（NK1R）0.01, P_（NPY）0.05, P_（NPY1R）0.05, P_（NPY2R）0.01）. Conclusion Moxibustion treatment may regulate the expression levels of proteins of S-100, SP, NK1R, NPY, NPY1R and NPY2R through warm stimulation, alleviate inflammatory response of colonic tissue, and repair impaired colonic mucosa, thus achieving the goal of treating Crohn＇s disease.