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Utilization of longitudinal ultrasound to quantify joint soft-tissue changes in a mouse model of posttraumatic osteoarthritis

Utilization of longitudinal ultrasound to quantify joint soft-tissue changes in a mouse model of posttraumatic osteoarthritis

作     者:Hao Xu Echoe M Bouta Ronald W Wood Edward M Schwarz Yongjun Wang Lianping Xing 

作者机构:Department of Orthopaedics Longhua Hospital Shanghai University of Traditional Chinese Medicine Department of Pathology and Laboratory Medicine Center for Musculoskeletal Research Department of Obstetrics and Gynecology University of Rochester Medical Center 

出 版 物:《Bone Research》 (骨研究(英文版))

年 卷 期:2017年第5卷第3期

页      面:228-234页

核心收录:

学科分类:1002[医学-临床医学] 100210[医学-外科学(含:普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基  金:supported by research grants from NIH,USA(AR048697 and AR063650 to LX) supported by research grants from NIH,USA(AR053459,AR056702,and AR061307 to EMS) supported by research grants from NIH,USA(AR048697 and AR063650 to LX) National Natural Science Foundation of China(81220108027 to YW and LX,81403418 to HX) 

主  题:OA PD USA Figure 

摘      要:To assess the utility of longitudinal ultrasound (US) to quantify volumetric changes in joint soft tissues during the progression of posttraumatic osteoarthritis (PTOA) in mice, and validate the US results with histological findings. A longitudinal cohort of 3-month-old wild-type C57BL/6 male mice received the Hulth-Telhag surgical procedure on right knee to induce ~OA, and sham surgery on their left knee as control. US scans were performed on both knees before, 2, 4, 6, and 8 weeks post-surgery. Joint space volume and Power-Doppler (PD) volume were obtained from US images via Amira software. A paraUel cross-sectional cohort of mice was killed at each US time point, and knee joints were subjected to histological analysis to obtain synovial soft-tissue area and OARSI scores. The correlation between US joint space volume and histological synovial soft-tissue area or OARSI score was assessed via linear regression analysis. US images indicated increased joint space volume in PTOA joints over time, which was associated with synovial inflammation and cartilage damage by histology. These changes started from 2 weeks post-surgery and gradually became more severe. No change was detected in sham joints. Increased joint space volume was significantly correlated with increased synovial soft-tissue area and the OARSI score (P 〈 0.001). PD signal was detected in the joint space of PTOA joints at 6 weeks post-surgery, which was consistent with the location of blood vessels that stained positively for CD31 and alpha-smooth muscle actin in the synovium. This study indicates that US is a cost-effective longitudinal outcome measure of volumetric and vascular changes in joint soft tissues during PTOA progression in mice, which positively correlates with synovial inflammation and cartilage damage.

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