Effects of MEK inhibitor U0126 on meiotic progression in mouse oocytes: microtuble organization, asymmetric division and metaphase Ⅱ arrest

作     者：CHAO TONG, HENG YU FAN, DA YUAN CHEN, XIANG FEN SONG, HEIDE SCHATTEN, QING YUAN SUNI 1 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China. 2 Department of Veterinary Pathobiology, University of Missouri-Columbia, MO 65211, USA CHAO TONG, HENG YU FAN, DA YUAN CHEN, XIANG FEN SONG, HEIDE SCHATTEN, QING YUAN SUNI 1 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China. 2 Department of Veterinary Pathobiology, University of Missouri-Columbia, MO 65211, USA

作者机构：1State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100080 China. E-mail: sunqy@*** 1State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100080 China. E-mail: sunqy@*** 1State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100080 China. E-mail: sunqy@*** 1State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100080 China. E-mail: sunqy@*** 2Department of Veterinary Pathobiology University of Missouri-Columbia MO 65211 USA. 1State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100080 China. E-mail: sunqy@*** 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2003年第13卷第5期

页      面：375-383页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：国家重点基础研究发展计划(973计划)(G1999055902) 国家自然科学基金(30225010,30170358) 中国科学院知识创新工程项目(KSCX2-SW-303,KSCX-IOZ-07) 

主　　题：kinases signal transduction oocyte development fertilization meiosis. 

摘      要：In this study we used U0126, a potent and specific inhibitor of MEK, to study the roles of MEK/ERK/p90rsk signaling pathway in the meiotic cell cycle of mouse oocytes. The phosphorylation of MAP kinase and p90rsk in the oocytes treated with 1.5 μM U0126 was the same as that in oocytes cultured in drug-free medium. With 1.5 μM U0126 treatment, the spindles appeared normal as they formed in oocytes, but failed to maintain its structure. Instead, the spindle lost one pole or elongated extraordinarily. After further culture, some oocytes extruded gigantic polar bodies （30 μm） that later divided into two small ones. Some oocytes underwent symmetric division and produced two equal-size daughter cells in which normal spindles formed. In oocytes with different division patterns, MAP kinase was normally phosphorylated. When the concentration of U0126 was increased to 15 mM, the phosphorylation of both MAPK and p90rsk were inhibited, while symmetric division was decreased. When incubating in medium c