Molecular and serological prevalence of Toxoplasma gondii in pregnant women and sheep in Egypt

作     者：Hany M.Ibrahim Azza H.Mohamed Ahmed A.El-Sharaawy Hend E.El-Shqanqery 

作者机构：Department of ZoologyFaculty of ScienceMenoufia UniversityShebin El-KomEgypt Clinical Pathology UnitNational Liver InstituteMenoufia UniversityEgypt 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2017年第10卷第10期

页      面：996-1001页

核心收录：

学科分类：090603[农学-临床兽医学] 1002[医学-临床医学] 100211[医学-妇产科学] 09[农学] 0906[农学-兽医学] 10[医学] 

主　　题：Toxoplasmosis RT-PCR ELISA Pregnant-women Sheep 

摘      要：Objective: To investigate molecular and serological prevalence of Toxoplasma gondii(***) in pregnant women and sheep in ***: Blood samples collected from healthy 364 pregnant women and 170 sheep were investigated for *** antibodies and parasitemia using highly specific and sensitive surface antigen(TgSAG2) based enzyme linked immunosorbent assay(ELISA)and real time-polymerase chain reaction(RT-PCR).Results: Overall prevalence of *** was 51.76%, 17.65% in sheep, 33.79%, 11.81%in pregnant women, using ELISA and RT-PCR *** differences in *** prevalence were observed on the basis of contact with cats or soil in pregnant women using either RT-PCR or *** pregnant women, a significant increase was detected in aged and those eating under-cooked mutton using simultaneous ELISA/***: Consumption of under-cooked infected mutton is an important source of human infection and the combination of the two assays provide accurate and precise data during infection.