Two-dimensional crystallization and preliminary electron crystallographic result of partially purified Fo from porcine mitochondria

作     者：ZHANG Xujia SUN Jingchuan YANG Hui LI Huilin HUANG Youguo YANG Fuyu 

作者机构：Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Life Science DivisionBerkeley National LaboratoryBerkeleyCA 94720USA 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2002年第47卷第4期

页      面：323-325页

核心收录：

学科分类：071011[理学-生物物理学] 0710[理学-生物学] 07[理学] 

基　　金：This work was supported by the Key Projectof Chinese Academy of Sciences (Grant No. KJ951-A1-601) the National Natural Science Foundation of China (Grant No. 39730130) the Fund of the Chinese Academy of Sciences to Zhang Xujia (Grant No. KSCX2-2-0 

主　　题：membrane protein proton channel F0 two-dimensional crystal electron microscopy. 

摘      要：After removal of cytoplasmic sector F1 from submitochondrial particles of F0F1-ATP synthase complex with guanidine hydrochloride, the transmembranc sector F0 was specifically extracted from the stripped membranes in the presence of detergent CHAPS and partially purified. Two-dimensional crystals were produced by the reconstitu-tion of the partially purified F0 into asolectin and microdi-alysis. The obtained crystals are able to diffract to 2 nm. The projection map of the negatively stained crystal shows that the crystal has p4212 symmetry, lattice constant, a = b = 14.4 nm. A unit cell contains four F0 molecules.