Rapid establishment of pure lines of silver carp (Hypophthalmichthys molirix) and bighead carp (Aristichthys nobilis)

作     者：WANG Zhongwei, YE Yuzhen, ZHOU Jianfeng and WU Qingjiang*(State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China) 

作者机构：Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol & Biotechnol Wuhan 430072 Peoples R China 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2004年第14卷第1期

页      面：61-64页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 09[农学] 0805[工学-材料科学与工程（可授工学、理学学位）] 071007[理学-遗传学] 090501[农学-动物遗传育种与繁殖] 0702[理学-物理学] 

基　　金：SupportedbytheNationalNaturalScienceFoundationofChina (GrantNo .3 983 0 3 00)andNational“TenthFive YearPlan”KeyProject(2 0 0 1BA5 0 5B0 50 8) 

主　　题：Hypophthalmichthys molirix Aristichthys nobilis RAPD genetic diversity pure line gynogenesis 

摘      要：The diversity of gynogenetic, artificial sex reversal and natural silver carp and bighead carp is examined using randomly amplified polymorphic DNA (RAPD) method. All of the 187 bands are obtained and 19 ( 10.16% ) of them are polymorphic in gynogenetic silver carp. Meanwhile 32 ( 15.61% ) out of 205 bands are polymorphic in control group. In gynogenetic bighead carp a total of 232 bands are identified and 11 ( 4.74% ) out of them are polymorphic, while 25 ( 10.37% ) out of 241 bands are polymorphic in control group. The genetic distance of four populations is calculated and it is 0.102 and 0.023 for gynogenetic silver carp and gynogenetic bighead carp respectively. The values of natural silver carp and bighead carp are 0.161 and 0.104 . From the UPGMA trees constructed based on genetic distance, the sex reversal individuals that match with the gynogenetic female individuals are picked out. A new breeding process of establishing a pure line is developed.