Construction of a recombinant plasmid harbouring the rhoptry protein 1 gene of Toxoplasma gondii and preliminary observations on DNA immunity
弓形虫侵入相关分子棒状体蛋白1基因重组质粒的克隆及其DNA免疫研究(英文)作者机构：中山医科大学寄生虫学研究所广州510089
出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)
年 卷 期：2001年第114卷第8期
页 面：54-57,107页
核心收录：
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学]
主 题：Toxoplasma gondii · rhoptry protein 1 · pcROP1 recombinant plasmid · cloning · DNA immunity
摘 要：Objective To observe the immune responses elicited in BALB/c mice by a DNA vaccine. A gene encoding rhoptry protein 1 (ROP1) from Toxoplasma gondii (T. gondii) was cloned into vector pcDNA3. Methods Amplifyied gene fragments coding for ROP1 from the genomic DNA of *** ZS2 were inserted into cloning vector, pUC18, and sub-cloned into pcDNA3. Mice were injected at a dosage of 100?μg recombinant plasmid DNA by intramuscular injection and boosted after 2 weeks. pcDNA3 and normal saline were used as control. 30, 50 and 70 days after the second immunization, NK cell activity, T lymphocyte proliferation and sub-clusters and serum IgG antibody were *** The specific gene fragment coding for ROP1 was amplified and a pcROP1 recombinant was constructed. At 30 days after immunization, the spleens of the mice were obviously enlarged evidently. NKC activity and the proliferation of spleen T lymphocytes seen on MTT assay were higher in pcROP1 group than in the controls. The number of CD4+ T cells exhibited no obvious increase compared with that of the control, but CD8+ T cells were obviously increased (P0.05). At 90 days after vaccination, the titer of IgG antibody in the serum of vaccinated mice was positive (1∶100). Conclusion pcROP1 was constructed and it could elicit both cellular and humoral immune responses in immunized mice.
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