Leishmania tarentolae: an alternative approach to the production of monoclonal antibodies to treat emerging viral infections

作     者：Joshua D Jones 

作者机构：Division of VirologyDepartment of PathologyUniversity of CambridgeTennis Court RoadCambridge CB21QPUK 

出 版 物：《Infectious Diseases of Poverty》 (贫困所致传染病（英文）)

年 卷 期：2015年第4卷第1期

页      面：83-87页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：I wish to thank Dr Ian Brierley(Division of Virology University of Cambridge)and Dr Nyarie Sithole(Department of Medicine University of Cambridge)for useful discussions and advice.JDJ is funded by a Wellcome Trust Infection and Immunity PhD Studentship 

主　　题：Antibody Ebola Emerging Expression Infection Leishmania Monoclonal Therapy 

摘      要：Background:Monoclonal antibody therapy has an important role to play as a post-exposure prophylactic and therapeutic for the treatment of viral infections,including emerging *** example,several patients of the present Ebola virus outbreak in West Africa were treated with ZMapp,a cocktail of three monoclonal antibodies which are expressed in Nicotiana ***:The majority of monoclonal antibodies in clinical use are expressed in mammalian cell lines which offer native folding and glycosylation of the expressed *** antibody expression in vegetal systems offers advantages over expression in mammalian cell lines,including improved potential for scale up and reduced *** this paper,I highlight the advantages of an upcoming protozoal system for the expression of recombinant antibody *** tarentolae offers a robust,economical expression of proteins with mammalian glycosylation patterns expressed in stable cell lines and grown in suspension *** advantages of this system make it particularly suited for use in developing ***:Given the potential importance of monoclonal antibody therapy in the containment of emerging viral infections,novel and alternative strategies to improve production must be explored.