Transfer of single dose of intravitreal injection of ranibizumab and bevacizumab into milk of sheep

作     者：Tugba Cakmak Argun Ozlem Yalcin Tok Levent Tok Gulsen Yilmaz Fatma Meric Yilmaz Alime Gunes Mehmet Argun Osman Butuner 

作者机构：Department of OphthalmologyHatay Dortyol State Hospital Faculty of Medicine OphthalmologySuleyman Demirel University Ministry of HealthAnkara HospitalBiochemistry Biochemistry DepartmentFaculty of MedicineYildirim Beyazit University The Management of Veterinary Service 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2017年第10卷第7期

页      面：1069-1075页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

主　　题：bevacizumab ranibizumab lactation milk vascular endothelial growth factor 

摘      要：AIM： To investigate whether single-dose intravitreal injections of bevacizumab and ranibizumab transfer into milk. METHODS： This study included lactating 12 sheep and a single 3-month old suckling lamb of each sheep. Two groups consisting of 6 sheep and their lambs were constituted; the ranibizumab group and the bevacizumab group before the administration of intravitreal injections, blood and milk samples were obtained from all sheep and, following the injections, blood and milk samples of all sheep and blood samples of all lambs were collected at regular time points. Serum and milk concentrations of bevacizumab and ranibizumab were measured using an enzyme-linked immunosorbent assay（ELISA） kit. The limit of determination was 0.9 ng/m L for bevacizumab and 0.62 ng/m L for ***： At 6h after intravitreal injections, bevacizumab concentration was above the limit of determination in the blood of all sheep. At 3wk, when the study was terminated, bevacizumab concentrations were high in 4 sheep. Even though bevacizumab concentrations in milk showed fluctuations, the drug transferred into the milk of all sheep at detectable concentrations. Ranibizumab drug concentrations in the blood and milk of sheep and those in the blood of lambs were below the limit of determination by the ELISA ***： This sheep model study demonstrate that intravitreal injection of ranibizumab, which did not transfer into the milk of sheep and suckling lambs, is safer than bevacizumab during lactation period.