A novel vWF A3-GPI modified EPCs to enhance its adhesion ability to collegen

作     者：Tan Hu Song Yaoming Jin Jun Zhao Xiaohui Huang Lan 

作者机构：Department of Cardiovascular Diseases Xinqiao Hospital Third Military Medical University Chongqing 400037 China 

出 版 物：《Journal of Medical Colleges of PLA(China)》 (中国人民解放军军医大学学报（英文版）)

年 卷 期：2011年第26卷第4期

页      面：191-203页

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 071003[理学-生理学] 

基　　金：Supported by the National Natural Science Foundation of China(30800479) 

主　　题：vWF A3-GPI Protein endothelial progenitor cells Collagen 

摘      要：Objective:To gain purified recombination protein rGPI-vWF A3 which can guide endothelial progenitor cells (EPCs) gathering to the internal surface of the injured vessels theoretically and analyze its bioactivity in vitro. Methods: The plasmid vector of vWF A3 fusion protein which contained GPI structure was constructed and recombination protein rGPI-vWF A3 was expressed and its bioactivity was analyzed by ELISA and FCM. Results: Fusion protein vWF A3-GPI was expressed and purified by immunoaffinity chromatography and it could stably anchored on the surface of EPCs and showed its biological activities of collagen conjunction. Conclusion: These findings represent a novel strategy in EPC transplantation treatment for vessel injury.