A highly efficient fluorescence-based switch-on detection method of 5-formyluracil in DNA
A highly efficient fluorescence-based switch-on detection method of 5-formyluracil in DNA作者机构：College of Chemistry and Molecular Sciences Key Laboratory of Biomedical Polymers of Ministry of Education The Institute for Advanced Studies Hubei Province Key Laboratory of AIlergy and Immunology Wuhan University Wuhan 430072 China
出 版 物：《Nano Research》 (纳米研究（英文版）)
年 卷 期：2017年第10卷第7期
页 面：2449-2458页
核心收录：
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 080801[工学-电机与电器] 081704[工学-应用化学] 0808[工学-电气工程] 07[理学] 08[工学] 0817[工学-化学工程与技术]
基 金：We thank the National Basic Research Program of China (Nos. 2012CB720600 and 2012CB720603) the National Natural Science Foundation of China (Nos. 21432008 91413109 and 81373256)
主 题：5-Formyluracil fluorescence phosphoramidite epigenetics
摘 要：The identification of hydroxylmethyl- and formylpyrimidines in genomic DNA was a landmark event in epigenetics. Numerous laboratories in related fields are investigating the biology of these and other nucleic acid modifications. However, limitations in the ability to detect and synthesize appropriate modifications are an impediment. Herein, we explored a remarkable development in the selective detection of 5-formyluracil in both single-stranded and double-stranded DNA under mild conditions. The ＂switch-on＂ specificity towards 5-formyluracil enabled a high signal-to-noise ratio in qualitatively and quantitatively detecting materials containing 5-formyluradl, which is not affected by the presence of abasic sites and 5-formylcytosine, the modified cytosine counterpart of 5-formyluracil. In summar~ the innoxiousness, convenience, and cost-effidency of the 5-formyluracil phosphoramidite synthetic routine would promote the understanding of the epi^enetic role of this natural thvmidine modification.
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