Genetic Stability of Cryopreserved Grapevine （Wtis vinifera L.） Genome by Vitrification Method
Genetic Stability of Cryopreserved Grapevine （Wtis vinifera L.） Genome by Vitrification Method作者机构：Food Science Coordination Research Center for Food and Development Hermosillo Sonora 83000 Mexico Food Technology of Plant Origin Coordination Research Center for Food and Development Hermosillo Sonora 83000 Mexico
出 版 物：《Journal of Agricultural Science and Technology(B)》 (农业科学与技术（B）)
年 卷 期：2016年第6卷第6期
页 面：380-386页
学科分类：08[工学] 0905[农学-畜牧学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090501[农学-动物遗传育种与繁殖]
主 题：Table grape axillary buds liquid nitrogen vitrification PVS2 inter-simple sequence repeats.
摘 要：Grapevine （Vitis spp.） is an economically important fruit crop worldwide. In Mexico, Sonora State leads the table grape production and exportation to international markets. In this regard, it is important to preserve the grape varieties during long time without phenotypical or genotypical changes. Cryopreservation is a good alternative, although it very often can induce changes in genome and phenotype. In this study, grapevine cv. ＂Flame Seedless＂ axillary buds were cryoprcserved by vitrification using the plant vitrification solution 2 （PVS2） and stored in liquid nitrogen （LN） for one hour, one week and one month, respectively. Genetic stability of buds cryopreserved under all treatments was evaluated using inter-simple sequence repeats （ISSR） markers. Ten ISSR primers were evaluated, but only two primers were possible to amplify distinct and reproducible bands with sizes between 300 bp and 2,000 bp. Different ISSR fragment patterns were recorded in cryopreserved buds as compared with control. These results suggest that cryopreservation by LN and vitrification-cryopreservation affect genetic stability in grapevine.
维普期刊数据库