TIMP-1 Production in Human Retinal Pigment Epithelial Cells after Laser Exposure

作     者：Alvin K.H.Kwok Timothy Y.Y.Lai Hin-Fai Yam Chi-Pui Pang 

作者机构：Department of Ophthalmology & Visual SciencesDepartment of Ophthalmology & Visual SciencesDepartment of Ophthalmology & Visual SciencesDepartment of Ophthalmology & Visual Sciences The Chinese University of Hong KongHong Kong SAR Department of OphthalmologyHong Kong Sanatorium and HospitalHong Kong SARThe Chinese University of Hong KongHong Kong SARThe Chinese University of Hong KongHong Kong SARThe Chinese University of Hong KongHong Kong SAR 

出 版 物：《Eye Science》 (眼科学报（英文版）)

年 卷 期：2005年第21卷第1期

页      面：31-37页

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

主　　题：TIMP-1 视网膜 上皮细胞 激光损伤 免疫细胞学 视色素 

摘      要：Purpose: To investigate changes in the production of tissue inhibitor of metalloproteinase type 1 (TIMP-1) by human retinal pigment epithelial (RPE) cells following argon laser ***: Human cultured ARPE19 cells were exposed to argon green laser at four different energy levels ranging from 60mW to 360mW. After laser exposure, the culture media were sampled at 0, 24, 72 and 144 hours for TIMP-1 concentration produced by the RPE cells. The levels of TIMP-1 in the cells treated with different laser energy levels were compared with a control group not exposed to laser *** for proliferating cell nuclear antigen (PCNA) was performed to detect any adverse effects on the RPE cells caused by laser ***: Immediately after laser exposure, the concentration of TIMP- 1 was not detectable. At 24 hours after laser exposure, the concentration of TIMP-1 increased significantly in RPE cells treated with 120mW and 240mW at 24 hours (P=0.006 and P=0.001respectively) compared with control cells. At 72 hours after treatment, RPE cells treated at 120mW, 240mW and 360mW demonstrated significantly increase in TIMP-1production compared with control (P=0.003, P 0.001 and P 0.001, respectively).No significant reduction in cell viability was observed following laser application as detected by PCNA ***: Our results demonstrated that early TIMP-1 production by RPE cells in cell cultures was enhanced following laser exposure.