MALDI Mass Spectrometry Analysis of Human Hemoglobin

作     者：PENG Wei-xian(Department of Physics, Jilin University, Changchun, 130023) 

作者机构：JILIN UNIV DEPT PHYS CHANGCHUN 130023 PEOPLES R CHINA 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：1996年第12卷第1期

页      面：44-49页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

主　　题：Laser Matrix Hemoglobin 

摘      要：Matrix-assisted laser desorption/ionisation(MALDI) mass spectra in matrices DHBs and ferulic acid have been taken and used to determine the molecular weight of one of the variants of human hemoglobin. A single laser operating at 355 nm was used to irradiate the sample droplet spots on a target for both desorption and ionisation. Ions were analyzed and detected by a reflectron time-of-flight mass spectrometer system. The mass values of α-chain and β-chain without heme group for a kind of human hemoglobin molecules were determined, which are 15275 Da and 16060Da respectively, with the accuracy of 0. 03%. The various factors influencing the resolution of mass spectrum were discussed.