ApoB-containing lipoproteins promote infectivity of chlamydial species in human hepatoma cell line
ApoB-containing lipoproteins promote infectivity of chlamydial species in human hepatoma cell line作者机构：Cambridge Theranostics LtdBabraham Research CampusBabrahamCambridgeCB2 4ATUnited Kingdom Gamaleya Institute for Epidemiology and Microbiology RAMS 18 Gamaleya Str.Moscow 123098Russia
出 版 物：《World Journal of Hepatology》 (世界肝病学杂志（英文版）（电子版）)
年 卷 期：2010年第2卷第2期
页 面：74-80页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by Cambridge Theranostics Ltd
主 题：ApoB-containing lipoproteins Chlamydial trachomatis Chlamydial pneumoniae Human hepatoma cell line Liver infection
摘 要：AIM:To evaluate the direct binding of two main chlamydial biovars(*** and ***) to plasma lipoproteins and its effect on chlamydial infection rate in human hepatoma cell line(HepG2 cells). METHODS:Murine plasma lipoproteins were fractionated and isolated using fast-performance liquid chromatography(FPLC),spotted on nitrocellulose membrane and incubated with chlamydial suspensions. Direct binding of chlamydial particles to lipoprotein fractions has been studied using lipopolysaccharide-specific antibodies in immuno-dot blot binding assay and immunoprecipitation *** protocol as well as flow cytometry analysis have been employed to study the infectivity rate of chlamydial species in HepG2 cells. RESULTS:Elementary bodies of both *** and *** bind ApoB-containing fractions of plasma *** binding becomes stronger when heat-denatured FPLC fractions are used, suggesting a primary role of apolipoproteins in interaction between chlamydial particle and lipoprotein. Both chlamydial biovars efficiently propagate in human hepatoma cell line-HepG2 cells even in serum free conditions forming late-stage inclusion bodies and releasing extracellular elementary *** of *** and *** with native ApoB-containing lipoproteins enhances the rate of chlamydial infection in HepG2 ***:A productive infection caused by C. trachomatis and *** may take place in human-derived hepatocytes revealing hepatic cells as possible target in chlamydial *** results may suggest the participation of lipoprotein receptors in the mechanism of attachment and/or entry of chlamydial particles into target cells.
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